Live-imaging fluorescent proteins in mouse embryos: multi-dimensional, multi-spectral perspectives
نویسندگان
چکیده
منابع مشابه
Live imaging of mouse embryos.
The development of the mouse embryo is a dynamic process that requires the spatial and temporal coordination of multiple cell types as they migrate, proliferate, undergo apoptosis, and differentiate to form complex structures. However, the confined nature of embryos as they develop in utero limits our ability to observe these morphogenetic events in vivo. Previous work has used fixed samples an...
متن کاملTopic Introduction Live Imaging of Mouse Embryos
The development of the mouse embryo is a dynamic process that requires the spatial and temporal coordination of multiple cell types as they migrate, proliferate, undergo apoptosis, and differentiate to form complex structures. However, the confined nature of embryos as they develop in utero limits our ability to observe these morphogenetic events in vivo. Previous work has used fixed samples an...
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Although we have advanced in our understanding of the molecular mechanisms intrinsic to the morphogenesis of chordate embryos, the question of how individual developmental events are integrated to generate the final morphological form is still unresolved. Microscopic observation is a pivotal tool in developmental biology, both for determining the normal course of events and for contrasting this...
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Visualization of object through acoustic waves is generally called as acoustical imaging. This concept contain noise source localization, ultrasonic non-destructive testing, medical ultrasound, underwater sonars, etc. In these conventional imaging techniques, frequency information has not yet utilized effectively, though shape, material and even structure behind the surface reflect on the frequ...
متن کاملThree-color imaging using fluorescent proteins in living zebrafish embryos.
The zebrafish embryo is especially valuable for cell biological studies because of its optical clarity. In this system, use of an in vivo fluorescent reporter has been limited to green fluorescent protein (GFP). We have examined other fluorescent proteins alone or in conjunction with GFP to investigate their efficacy as markers for multi-labeling purposes in live zebrafish. By injecting plasmid...
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ژورنال
عنوان ژورنال: Trends in Biotechnology
سال: 2009
ISSN: 0167-7799
DOI: 10.1016/j.tibtech.2009.02.006